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2022-05-09

陳坤麟老師研發高敏感度SARS-CoV-2快篩

In the past decades, different diseases and viruses, such as Ebola, MERS and COVID-19, impacted the human society and caused huge cost in different fields. With the increasing threat from the new or unknown diseases, the demand of rapid and sensitive assay method is more and more urgent. In this work, we developed a magneto-optical biochip based on the Cotton–Mouton effect of γ-Fe2O3@Au core/shell magnetic nanoparticles. We performed a proof-of-concept experiment for the detection of the spike glycoprotein S of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The assay was achieved by measuring the magneto-optical Cotton–Mouton effect of the biochip. This magneto-optical biochip can not only be used to detect SARS-CoV-2 but also can be easily modified for other diseases assay. The assay process is simple and the whole testing time takes only 50 min including 3 min for the CM rotation measurement. The detection limit of our method for the spike glycoprotein S of SARS-CoV-2 is estimated as low as 0.27 ng/mL (3.4 pM).
 
在過去的幾十年裡,不同的疾病和病毒,如伊波拉、中東呼吸綜合症和 COVID-19,對人類社會產生了影響,並在不同領域造成了巨大的損失。隨著新的或未知疾病的威脅越來越大,對快速靈敏的檢測方法的需求也越來越迫切。在這項工作中,我們開發了一種基於γ-Fe2O3@Au核/殼磁性奈米粒子克頓-莫頓效應的磁光生物晶片。中興大學物理系 陳坤麟教授研究團隊進行了一項概念驗證實驗,用於檢測新型冠狀病毒SARS-CoV-2 的刺突醣蛋白S。該測定是通過測量生物芯片的磁光克頓-莫頓效應來實現的。這種磁光生物芯片不僅可以用於檢測 SARS-CoV-2,還可以輕鬆修改用於其他疾病檢測。測定過程簡單,整個測試時間僅需50分鐘,其中3分鐘用於磁光克頓-莫頓旋轉角的測量。陳坤麟教授研究團隊的方法對SARS-CoV-2的刺突醣蛋白S的檢測極限為0.27 ng/mL (3.4 pM),優於目前多數的檢測法。

本研究發表於: Chen, KL., Yang, ZY. & Lin, CW. A magneto-optical biochip for rapid assay based on the Cotton–Mouton effect of γ-Fe2O3@Au core/shell nanoparticles. J Nanobiotechnol 19, 301 (2021). https://doi.org/10.1186/s12951-021-01030-z
 
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